Page Index - MetabolicEngineeringGroupCBMA/MetabolicEngineeringGroupCBMA.github.io GitHub Wiki

703 page(s) in this GitHub Wiki:

• Home
• Basics
• Commonly used protocols
• Cultivating the yeast Saccharomyces cerevisiae for any purpose
• The Yeast Pathway Kit
• Gel electrophoresis
• Plasmid DNA from E. coli
• DNA
• PCR
• DNA extraction & colony PCR
• Transformation
• E. coli
• S. cerevisiae
• Yeast cell (protein) extract from S. cerevisiae
• RNA
• Miscellaneous Methods
• E. coli strains
• E. coli media
• Yeast media
• Stock solutions
• Miscellaneous
• 10X Taq Buffer with (NH4)2SO4
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• 2x PCR mastermix
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• 2xPCR mastermix
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• 6x DNA loading buffer
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• Acknowledgement
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• addgene_standard_primers
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• Agar
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• agarose gel casting
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• alkaline lysis plasmid mini prep
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• amc
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• amino acid drop out mix
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• amino acids
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• Ampicillin
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• ampicillin
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• anabolic
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• antibiotics
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• ApE
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• assay
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• autoclaving
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• Bachman
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• Bio Rad Mini Sub Cell
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• Bloom verbs
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• boiling miniprep
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• bradford
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• broken links output
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• cake
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• Calcium Competent Cells
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• catabolic
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• catalase lab
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• catalase lab pt
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• Chloramphenicol plasmid amplification
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• chokladbollar
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• clip art
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• Cloning documentation workshop
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• colab
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• Congresses
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• Consumo de Glicose
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• Counting Yeast Cells Using a hemocytometer
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• cracking gel
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• CRISPr with plasmid pRCC K
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• cuvette
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• ddH2O
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• Delft
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• delft
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• dH2O
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• DH5α
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• disintegration protocol
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• disruptor genie
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• DNA
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• DNA loading buffer
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• dnaudit
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• DNS
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• DNS pt
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• dNTPs
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• Documenting a genetic construct
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• dsIUPAC
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• EDTA
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• EGB23
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• EGB24
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• EGB25
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• electrophoresis
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• elitech datareader
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• emoji
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• Engenharia Genética e Bioinformática
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• English
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• enzyme
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• enzyme activity
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• Enzyme activity exercise
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• Eppendorf tube
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• Erlenmeyer
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• etbr
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• ethanol precipitation
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• eurofins_standard_primers
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• Extraction of chromosomal DNA with PCA (phenol:chloroform:isoamylalcohol)
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• FALCON
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• FASII
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• FASTA
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• FastPrep
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• fatval
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• Fisksoppa
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• GBM23
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• Genbank
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• genbank reference
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• GENESYS20
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• Genetic nomenclature
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• Geneticin
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• geneticin
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• GenoSmart
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• getting started
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• glass bead plating
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• glassware
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• Glucose
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• Glucose Consumption
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• Glucose Consumption pt
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• glycolysis
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• GMB21
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• Greek letters
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• GRS Plasmid Purification Kit
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• High competent cells from liquid culture
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• High Efficiency Yeast Transformation Protocol
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• homeostasis
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• homologous recombination
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• How many times can I reuse electrophoresis buffer?
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• How to mark your material
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• how to pipette correctly
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• How to prepare for the computer test
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• how to read a paper
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• how_to_plan_a_software_project
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• Hygromycin
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• illustrations
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• in silico assembly of a pYPKa vector
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• in silico assembly of a TU vector
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• in silico assembly of pTA1_TDH3_ATF1_PGI1
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• in silico assembly of pTA1_TDH3_ScATF1_PGI1
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• in silico assembly of pYPKa_A_ATF1
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• In vivo assembly in yeast
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• indonesisk kycklinggryta
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• IPTG
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• IUPAC
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• journal club
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• kakvåfflor
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• kanelbullar
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• KanMX4
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• KCl
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• kycklingsoppa
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• LAB7️⃣ PL3 plasmid rescue, E. coli transformation
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• ladders
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• lamp
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• LB
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• LB amp
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• LB kan
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• LB24
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• LBM big vortex
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• LBpowder
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• LBx6
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• LGM
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• LiAc Borate
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• LIB20
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• Ligase buffer
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• LIGATION
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• ligation
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• Ligation mastermix
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• linear regression
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• linkers
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• lyticase
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• M9
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• maintaining lab notes
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• material
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• mec
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• Merlin
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• metabolic maps
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• MG7α
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• MgCl2
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• MgSO4
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• Microcentrifuge
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• microscope
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• Microwave Yeast Colony PCR
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• microwave yeast colony pcr
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• midiprep
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• Midori Green DNA loading buffer (LBx2wMidori)
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• minitron
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• mRNA extraction
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• MultiTube_Vortexer_Multi Tube_Vortexer
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• NaCl
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• NanoDrop
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• NaOH
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• NaOH Yeast Colony PCR
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• NZYMiniprep
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• P40Y20
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• panasonic
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• parafilm
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• Pathway assembly with the Yeast Pathway Kit
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• PBS
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• PCR clean up
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• pdf
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• PEG LiAc ssDNA
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• people
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• petri dish grid
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• phone dir
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• pipetting
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• plasmid miniprep
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• plasmid rescue
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• plasmid_maps
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• PLS
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• potato broth
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• practical considerations
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• Preparation of Penn State DNA ladders (pPSU)
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• Preparation of yeast cell extracts with Y PER
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• primer design
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• primer_design
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• primers
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• pTA1_FASIIb_pLann
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• pTAx assembly strategy
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• pUCmu_1669_bp_cdseguid=jVV0rZwWovd7vwx7XZiAakfYmQE.txt
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• Purifying and testing recombinant Taq DNA polymerase
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• pYPKa cloning protocol
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• pYPKa cloning protocoll
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• python
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• Rapid isolation of yeast total DNA
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• rectifier
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• Render Jupyter notebook in your browser
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• Replacing Notepad
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• restriction digestion
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• SD
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• SDS
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• search
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• SEM (Inoue) competent cells
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• sequence_formats
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• sequencing
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• SimplyCloning
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• snik short
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• SOB
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• SOC
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• software
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• speedvac
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• square petri dish
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• standard pcr protocol
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• Standard protocol for preparing yeast cells
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• standard_primers
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• stock solutions
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• Strain information
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• Streak for single colonies
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• Sucrose
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• Super Broth
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• SuperBroth
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• symbol
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• Tables
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• TAE
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• Taq purification
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• TB
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• TBE
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• TE
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• Teaching
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• Terrific Broth
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• Tetracycline
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• tetracycline
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• The Yeast Pathway Kit
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• todo
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• TP01
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• TP02
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• TP02_Visualizing_linear_and_circular_DNA_with_ApE
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• TP02_Visualizing_linear_and_circular_DNA_with_ApE
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• TP03
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• TP03_In silico_sub_cloning_using_ApE
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• TP04
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• TP04_Manual_PCR_simulation\TP04_Manual_PCR_simulation
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• TP05
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• TP05
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• TP05_Primer_design_and_melting_temperature_calculations_for_PCR_primers\TP05_Primer_design_and_melting_temperature_calculations_for_PCR_primers
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• TP06
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• TP06
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• TP06
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• TP06_How_to_download_sequences_from_Genbank\TP06_How_to_download_sequences_from_Genbank
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• TP07
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• TP07_Genbank_and_the_Genbank_flat_file_format\TP07_Genbank_and_the_Genbank_flat_file_format
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• TP08
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• TP08_Saccharomyces_Genome_Database\TP08_Saccharomyces_Genome_Database
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• TP09
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• TP09
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• TP09_Cloning_by_HR_and_Gibson_assembly\TP09_Cloning_by_HR_and_Gibson_assembly
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• TP10
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• TP10_Searching_databases_using_BLAST\TP10_Searching_databases_using_BLAST
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• TP11_fungal_protein_BLAST\TP11_fungal_protein_BLAST
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• TP12
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• TP12_Restriction_mapping2\TP12_Restriction_mapping2
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• TP13
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• TP13_Agarose_gel_electrophoresis\TP13_Agarose_gel_electrophoresis
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• TP14_how_to_take_the_bioinformatics_test_on_your_own_computer\TP14_how_to_take_the_bioinformatics_test_on_your_own_computer
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• TP16_Sequence_comparison_using_dotlet\TP16_Sequence_comparison_using_dotlet
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• TP17_Picking_primers_for_cloning_and_sequencing_with_the_webserver_Primer3Plus\TP17_Picking_primers_for_cloning_and_sequencing_with_the_webserver_Primer3Plus
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• TP18
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• TP18_assembly_of_the_p416TEF_vector_from_a_published_cloning_strategy\TP18_assembly_of_the_p416TEF_vector_from_a_published_cloning_strategy
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• TP19_Picking_primers_with_ApE\TP19_Picking_primers_with_ApE
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• TP20_Assembly_of_pTAx_vectors\TP20_Assembly_of_pTAx_vectors
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• TP21
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• TP21_Automatic_PCR_product_simulation_using_WebPCR_v004\TP21_Automatic_PCR_product_simulation_using_WebPCR_v004
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• TP22_Assembly_of_a_yeast_deletion_cassette\TP22_Assembly_of_a_yeast_deletion_cassette
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• TP23
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• TP23_Design_of_a_c terminal_GFP_fusion_protein_expression_construct_using_pUG35\TP23_Design_of_a_c terminal_GFP_fusion_protein_expression_construct_using_pUG35
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• TP24_small_scale_assembly_of_DNA_sequence_reads_v001\TP24_small_scale_assembly_of_DNA_sequence_reads_v001
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• TP25
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• TP25_GoldenGateCloning\TP25_GoldenGateCloning
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• TP26 Simply Cloning
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• TP26_Simply_Cloning\TP26_Simply_Cloning
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• TP27_EduBlocks_Python3\TP27_EduBlocks_Python3
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• TP28_Metabolic_Yield_Calculations\TP28_Metabolic_Yield_Calculations
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• TP31_Primer_design_and_cloning_genetic_elements_in_YPK\TP31_Primer_design_and_cloning_genetic_elements_in_YPK
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• TP33\TP33
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• TP34_Assembly_of_a_TU_using_YPK\TP34_Assembly_of_a_TU_using_YPK
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• TP35
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• TP35 Chemical Thermodynamics
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• TP35 Chemical Thermodynamics
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• TP35 Chemical Thermodynamics pt
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• TP35 Chemical Thermodynamics pt
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• TP35pt
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• TP36
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• TP36 Metabolism
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• TP36 Metabolism
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• TP36 Metabolism pt
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• TP36 Metabolism pt
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• TP36pt
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• TP37
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• TP37 Metabolism II
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• TP37pt
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• TPs
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• trafo prepare frozen competent yeast
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• Transcription Unit cloning protocol
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• transesterification
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• Transform frozen competent yeast cells
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• Transforming Frozen Competent E. coli
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• TrisHCl
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• Turner
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• Tween Myristic acid
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• Untitled
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• vaccuum manifold
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• vortex
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• weighing boat
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• writing
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• XL1 blue
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• xyzzy
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• Y2H_by_mating
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• Yeast and bacterial freezer stocks
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• yeast cell extract
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• Yeast cell fixation with formaldehyde
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• yeast colony pcr
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• Yeast DNA isolation with Lyticase or Zymolyase
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• Yeast genomic DNA extraction with LiOAc SDS
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• Yeast transformation GBM23
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• Yeast transformation GBM24
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• yeast transformation short
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• YNB
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• YPA
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• YPArabinose
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• YPD
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• YPDlight
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• YPDpowder
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• YPE
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• YPFD
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• YPGal
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• YPGES
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• ypk_restriction_enzymes
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