Agar - MetabolicEngineeringGroupCBMA/MetabolicEngineeringGroupCBMA.github.io GitHub Wiki

If you work with several different media such as YPD, LB, SOB and SOC liquid and solid it might pay off to separate the solutions to a smaller set that can be combined to form each medium.

For this reason, you can prepare a sterile >twice concentrated agar solution in a Schott flask. These can be kept at room temperature almost indefinitely.

For 500 mL solid medium (~20 plates):

• 1 L Schott flask
• 10 g Agar
• ~200 mL water
• Optional a magnetic stirrer bar Sterilize by autoclaving.

Add water and media components to 500 mL and heat in a microwave oven (5-10 min) until the agar has been dissolved.