SOB - MetabolicEngineeringGroupCBMA/MetabolicEngineeringGroupCBMA.github.io GitHub Wiki
Super Optimal Broth. For 1 L medium, mix the following components in a Schott bottle:
- 20 g Tryptone or Peptone
- 5 g Yeast extract
- 0.5 g NaCl
- 1 mL KCl 2.5 M
Autoclave, cool to room temperature and add:
Alternatively, the Tryptone and Yeast Extract can be autoclaved and the rest of the components can be added from sterile stock solutions: