SOB - MetabolicEngineeringGroupCBMA/MetabolicEngineeringGroupCBMA.github.io GitHub Wiki

Super Optimal Broth. For 1 L medium, mix the following components in a Schott bottle:

  • 20 g Tryptone or Peptone
  • 5 g Yeast extract
  • 0.5 g NaCl
  • 1 mL KCl 2.5 M

Autoclave, cool to room temperature and add:

  • 5 ml of a sterile 2M MgCl2
  • 5 mL of sterile 2M MgSO4

Alternatively, the Tryptone and Yeast Extract can be autoclaved and the rest of the components can be added from sterile stock solutions:

  • 1 mL/L 250 g/L NaCl
  • 1 mL/L 2.5 M KCl
  • 5 ml/L of a sterile 2M MgCl2
  • 5 mL/L of sterile 2M MgSO4