geneticin - MetabolicEngineeringGroupCBMA/MetabolicEngineeringGroupCBMA.github.io GitHub Wiki

Geneticin(G418) stock solution 200 mg/mL (x 1000).

Geneticin (Gibco's brand name) or G418 (generic name) is used to select for yeast that have the KanMX resistance marker. We make G418 stock solution at 200 mg/ml (1000x) and use a final concentration of 200 μg/ml in solid or liquid media. The working solution is stored in 1 mL aliquots marked with a capital "G" at -20°C. Put these tube is the box marked "Antibiotics" in the -20 freezer.

Goldstein, A.L., McCusker, J.H., 1999. Three new dominant drug resistance cassettes for gene disruption in Saccharomyces cerevisiae. Yeast 15, 1541–1553.