YPDpowder - MetabolicEngineeringGroupCBMA/MetabolicEngineeringGroupCBMA.github.io GitHub Wiki

YPD medium can be mixed in dry form and used to quickly prepare YPD using a microwave oven. This can be a practical way of storing a YPD stock in dry form.

For 2.5 L final medium:

• 25 g yeast extract
• 50 g peptone
• 50 g glucose

For 500 ml liquid medium, take 25 g of the mix and dissolve in 500 mL tap water add 10g agar for plates. Use a 1 L Schott bottle and run for 10 min at full power in a microwave oven with the cap shut tightly.

Protect the powder from moisture or the glucose will turn it into a solid paste.