Hygromycin - MetabolicEngineeringGroupCBMA/MetabolicEngineeringGroupCBMA.github.io GitHub Wiki
Hygromycin B
Standard concentration for selection in S. cerevisiae is 300 µg/ml according to Goldstein 1999
Formedium sell this here.
Stock solution 300 mg/mL (x 1000)
- Weigh 3 g Hygromycin B powder in a Falcon 15 mL tube.
- Add water to the 10 mL mark and vortex. Add more water if necessary.
- Filter through 0.22 µM membrane with a large syringe.
- store at -20 in 1 ml aliquots.