Strain information - MetabolicEngineeringGroupCBMA/MetabolicEngineeringGroupCBMA.github.io GitHub Wiki
Information on the CEN.PK strains can be found in:
Entian, Karl-Dieter, and Peter Kötter. 2007. “25 Yeast Genetic Strain and Plasmid Collections.” In Methods in Microbiology, ed by. Ian Stansfield and Michael Stark Jr, 36:629–666. Academic Press.
| Name | Mating type | URA | HIS | LEU | TRP | Other | |
|---|---|---|---|---|---|---|---|
| CENPK113-7D | MATa | URA3 | HIS3 | LEU2 | TRP1 | MAL2-8c | SUC2 |
| CENPK113-5D | MATa | ura3-52 | HIS3 | LEU2 | TRP1 | MAL2-8c | SUC2 |
| CENPK113-7A | MATa | URA3 | his3-Δ1 | LEU2 | TRP1 | MAL2-8c | SUC2 |
| CENPK111-32D | MATa | URA3 | HIS3 | leu2-3_112 | TRP1 | MAL2-8c | SUC2 |
| CENPK113-3C | MATa | URA3 | HIS3 | LEU2 | trp1-289 | MAL2-8c | SUC2 |
| CENPK113-11C | MATa | ura3-52 | his3-Δ1 | LEU2 | TRP1 | MAL2-8c | SUC2 |
| CENPK102-3A | MATa | ura3-52 | HIS3 | leu2-3_112 | TRP1 | MAL2-8c | SUC2 |
| CENPK113-9D | MATa | ura3-52 | HIS3 | LEU2 | trp1-289 | MAL2-8c | SUC2 |
| CENPK111-9A | MATa | URA3 | his3-Δ1 | leu2-3_112 | TRP1 | MAL2-8c | SUC2 |
| CENPK113-11A | MATa | URA3 | his3-Δ1 | LEU2 | trp1-289 | MAL2-8c | SUC2 |
| CENPK111-27B | MATa | URA3 | HIS3 | leu2-3_112 | trp1-289 | MAL2-8c | SUC2 |
| CENPK2-1C | MATa | ura3-52 | his3-Δ1 | leu2-3_112 | trp1-289 | MAL2-8c | SUC2 |
| CENPK102-5B | MATa | ura3-52 | his3-Δ1 | leu2-3_112 | TRP1 | MAL2-8c | SUC2 |
| CENPK113-6B | MATa | ura3-52 | HIS3 | leu2-3_112 | trp1-289 | MAL2-8c | SUC2 |
| CENPK111-61A | MATalpha | ura3-52 | his3-Δ1 | leu2-3_112 | TRP1 | MAL2-8c | SUC2 |
| CENPK110-10C | MATalpha | URA3 | his3-Δ1 | LEU2 | TRP1 | MAL2-8c | SUC2 |
The CEN.PK strains were developed for:
- high growth rates in complete and defined media under aerobic and anaerobic conditions in batch, fed-batch and continuous culture
- high mating and sporulation efficiency
- high spore viability
- high transformation efficiency
- good single-cell formation (no flakiness or flocculation)
All CEN.PK strains contain the SUC2 gene for sucrose utilization and the MAL2-8c gene for maltose utilization. MAL2-8c is a dominant mutant allele that causes a partially constitutive (not requiring maltose as an inducer) but still glucose-repressible MAL2 gene expression.
CEN.PK strains are hypersensitivity to sodium and lithium ions (Daran-Lapujade et al. 2009). The CEN.PK strains have a weak cAMP response compared to some naturally occurring yeasts (Dumortier et al. 2000) due to the adenylate cyclase Cyr1(YJL005W) K1876M variant. This is a property shared with some other laboratory strains. The genome of CEN.PK113-7D was sequenced (Nijkamp et al. 2012).
Daran-Lapujade, Pascale, Jean-Marc Daran, Marijke A. H. Luttik, Marinka J. H. Almering, Jack T. Pronk, and Peter Kötter. 2009. “An Atypical PMR2 Locus Is Responsible for Hypersensitivity to Sodium and Lithium Cations in the Laboratory Strain Saccharomyces Cerevisiae CEN.PK113-7D.” FEMS Yeast Research 9 (5) (August): 789–792.
Dumortier, Françoise, Mieke Vanhalewyn, Gilda Debast, Sonia Colombo, Pingsheng Ma, Joris Winderickx, Patrick Van Dijck, and Johan M. Thevelein. 2000. “A Specific Mutation in Saccharomyces Cerevisiae Adenylate Cyclase, Cyr1K1876M, Eliminates Glucose- and Acidification-Induced cAMP Signalling and Delays Glucose-Induced Loss of Stress Resistance.” International Journal of Food Microbiology 55 (1) (April 10): 103–107.
Nijkamp, Jurgen F., Marcel van den Broek, Erwin Datema, Stefan de Kok, Lizanne Bosman, Marijke A. Luttik, Pascale Daran-Lapujade, et al. 2012. “De Novo Sequencing, Assembly and Analysis of the Genome of the Laboratory Strain Saccharomyces Cerevisiae CEN.PK113-7D, a Model for Modern Industrial Biotechnology.” Microbial Cell Factories 11 (March 26): 36.