This protocol was adapted from Lõoke M, Kristjuhan K, Kristjuhan A. Extraction of genomic DNA from yeasts for PCR-based applications. Biotechniques 2011;50:325–8.. According to the authors, it allows amplifying up to 3500 bp PCR products from yeast genomic DNA. It appears to be a popular protocol since it has many publications referring to it. This protocol is one of the most cited in literature. This is an indication that it works well.

1. Spin down 200 μL liquid yeast culture[^1].
2. Suspend cells in 100 μL LiOAc (200 mM) containing 1% SDS.

proceed to point 2 below

1. Pick one yeast colony from a plate.

2. Suspend cells in 100 μL LiOAc (200 mM) with 1% SDS.

3. Incubate for 15 min on a bath of recently boiled water. Original protocol states 5-15 min at 70°C.

4. Add 300 μL 96–100% ethanol, then mix by brief vortexing.

5. Spin down DNA and cell debris at 15,000×g or the max speed for 3 min.

6. Remove supernatant by decanting or pipetting.

7. Add 1 mL 70% ethanol. Vortex briefly.

8. Spin at the maximum speed that the centrifuge allow for 30s.

9. Remove ethanol by decanting or pipetting.

10. Dissolve pellet in 100 μL TE, H2O or Elution buffer from a miniprep kit.

11. Spin down cell debris for 15 s or more at maximum speed.

12. Use 1 μL supernatant for PCR.

13. Store the chromosomal DNA at -20°C if it is not to be used immediately.

short version