SimplyCloning

A series of ten youtube videos by Andriy Nemirov describing a sub cloning experiment from planning to verification of the final construct.

Simply Cloning - Chapter 1 - Planning 12:27

Simply Cloning - Chapter 2 - PCR 06:39

Simply Cloning - Chapter 3 - Vector Restriction Digest 02:52

Simply Cloning - Chapter 4 - Gel Purification 11:47

Simply Cloning - Chapter 5 - Insert Restriction Digest 02:55

Simply Cloning - Chapter 6 - Ligation 06:10

Simply Cloning - Chapter 7 - Transformation 08:02

Simply Cloning - Chapter 8 - Plasmid Miniprep 06:05

Simply Cloning - Chapter 9 - Clone Verification 05:20

Simply Cloning - Chapter 10 - Clone Library 02:48

Questions

Question 1: What is the cdseguid of the pSAT6-Bar vector?

Question 2: Why does he run the gel in Chapter 4?