Protocol for making x 10 times concentrated YNB (Yeast Nitrogen Base) solution

The final concentration of YNB in media depends on the type of YNB

Component	C (g/L)	Ammonium sulfate	Amino acids
YNB w/o amino acids w ammonium sulfate	6.7	✅	✅
YNB w amino acids w ammonium sulfate	6.7	✅	❌
YNB w/o ammonium sulfate	1.7	❌	❌

YNB w amino acids has:

• L-Histidine HCl
• DL-Methionine
• DL-Tryptophan

YNB w ammonium sulfate has 5 g/L ammonium sulfate.

10 x YNB w ammonium sulfate w amino acids

Dissolve 13.4 g YNB w (NH4)2SO4 in 200 mL water. Sterilize by autoclaving.

10 x YNB w/o ammonium sulfate

Dissolve 13.4 g YNB w/o (NH4)2SO4 in 200 mL water. Sterilize by autoclaving.