TB - MetabolicEngineeringGroupCBMA/MetabolicEngineeringGroupCBMA.github.io GitHub Wiki

• TB PROTOCOL[EXP:true]

Terrific Broth

http://www.thelabrat.com/protocols/TerrificBroth.shtml

Add the following to 800ml distilled H2O 12g Tryptone 24g Yeast extract 4ml Glycerol

2. Adjust to 900ml with distilled H2O
3. Sterilize by autoclaving
4. Allow to cool to room temperature
5. Adjust volume to 1000ml with 100ml of a filter sterilized solution of 0.17M KH2PO4 and 0.72M K2HPO4

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Terrific Broth http://cshprotocols.cshlp.org/content/2006/1/pdb.rec8620

Deionized H2O, to 900 mL Tryptone, 12 g Yeast extract, 24 g Glycerol, 4 mL

Shake until the solutes have dissolved and then sterilize by autoclaving for 20 min at 15 psi (1.05 kg/cm2) on liquid cycle. Allow the solution to cool to 60°C or less, and then add 100 mL of a sterile solution of 0.17 M KH2PO4, 0.72 M K2HPO4. (This solution is made by dissolving 2.31 g of KH2PO4 and 12.54 g of K2HPO4 in 90 mL of H2O. After the salts have dissolved, adjust the volume of the solution to 100 mL with H2O and sterilize by autoclaving for 20 min at 15 psi [1.05 kg/cm2] on liquid cycle.)