TPs - MetabolicEngineeringGroupCBMA/MetabolicEngineeringGroupCBMA.github.io GitHub Wiki

Best practices:

1. Keep all files and software like ApE in one folder
2. Use Editpad Online text editor (or similar) to collect responses to the questions

• TP01_Calculating_checksums_using_seguid_calculator

• TP02_Visualizing_linear_and_circular_DNA_with_ApE

• TP03_In-silico_sub_cloning_using_ApE

• TP04_Manual_PCR_simulation

• TP05_Primer_design_and_melting_temperature_calculations_for_PCR_primers

• TP06_How_to_download_sequences_from_Genbank

• TP07_Genbank_and_the_Genbank_flat_file_format

• TP08_Saccharomyces_Genome_Database

• TP09_Cloning_by_HR_and_Gibson_assembly

• TP10_Searching_databases_using_BLAST

• TP11_Fungal_protein_BLAST

• TP12_Restriction_mapping

• TP13_Agarose_gel_electrophoresis

• TP14_how_to_take_the_bioinformatics_test_on_your_own_computer

• TP15_Sanger_sequencing

• TP16_Sequence_comparison_using_dotlet

• TP17_Picking_primers_for_cloning_and_sequencing_with_Primer3Plus

• TP18_Reproducing_a_cloning_strategy_from_literature

• TP19_Picking_primers_with_ApE

• TP20_Assembly_of_pTAx_vectors

• TP21_Automatic_PCR_product_simulation_using_WebPCR

• TP22_Assembly_of_a_yeast_gene_deletion_cassette

• TP23_Design_of_a_c-terminal_GFP_fusion_protein_expression_vector

• TP24_Small_scale_assembly_of_sequence_reads

• TP25_GoldenGateCloning

• TP26_Simply_Cloning

• TP27_EduBlocks_Python3

• TP28_Metabolic_Yield_Calculations

• TP29_GATEWAY_recombinational_cloning

• TP30_CRISPr-CAS9

• TP31_Primer_design_and_cloning_genetic_elements_in_YPK

• TP32_Growth_curves_and_growth_rate_calculations

• TP33_DNA_calculations

• TP34_Assembly_of_a_TU_using_YPK

• TP35 Chemical Thermodynamics

• TP36 Metabolism

• TP37 Metabolism II