TPs - MetabolicEngineeringGroupCBMA/MetabolicEngineeringGroupCBMA.github.io GitHub Wiki Best practices: Keep all files and software in one folder Use Editpad Online text editor TP01_Calculating_checksums_using_seguid_calculator TP02_Visualizing_linear_and_circular_DNA_with_ApE TP03_In-silico_sub_cloning_using_ApE TP04_Manual_PCR_simulation TP05_Primer_design_and_melting_temperature_calculations_for_PCR_primers TP06_How_to_download_sequences_from_Genbank TP07_Genbank_and_the_Genbank_flat_file_format TP08_Saccharomyces_Genome_Database TP09_Cloning_by_HR_and_Gibson_assembly TP10_Searching_databases_using_BLAST TP11_Fungal_protein_BLAST TP12_Restriction_mapping TP13_Agarose_gel_electrophoresis TP14_how_to_take_the_bioinformatics_test_on_your_own_computer TP15_Sanger_sequencing TP16_Sequence_comparison_using_dotlet TP17_Picking_primers_for_cloning_and_sequencing_with_Primer3Plus TP18_Reproducing_a_cloning_strategy_from_literature TP19_Picking_primers_with_ApE TP20_Assembly_of_pTAx_vectors TP21_Automatic_PCR_product_simulation_using_WebPCR TP22_Assembly_of_a_yeast_gene_deletion_cassette TP23_Design_of_a_c-terminal_GFP_fusion_protein_expression_vector TP24_Small_scale_assembly_of_sequence_reads TP25_GoldenGateCloning TP26_Simply_Cloning TP27_EduBlocks_Python3 TP28_Metabolic_Yield_Calculations TP29_GATEWAY_recombinational_cloning TP30_CRISPr-CAS9 TP31_Primer_design_and_cloning_genetic_elements_in_YPK TP32_Growth_curves_and_growth_rate_calculations TP33_DNA_calculations TP34_Assembly_of_a_TU_using_YPK TP35 Chemical Thermodynamics TP36 Metabolism TP37 Metabolism II