TAE - MetabolicEngineeringGroupCBMA/MetabolicEngineeringGroupCBMA.github.io GitHub Wiki

TAE buffer is commonly prepared as a 50× stock solution for laboratory use.

This stock solution can be diluted 49:1 with water to make a 1× working solution.

Name	Per 1 mole	50x solution	50×	1× solution
Tris base	121.1 g/L	2 M	242.2 g/L	40 mM
acetic acid	57.1 ml/L	1 M	57.1 ml/L	20 mM
EDTA disodium salt dihydrate	372.24 g/L	50 mM	18.612 g/L	1 mM
IMPORTANT! Use Tris base, not Tris-HCl.