ApE - MetabolicEngineeringGroupCBMA/MetabolicEngineeringGroupCBMA.github.io GitHub Wiki
ApE (A Plasmid Editor) is a freely available DNA Manipulation and visualization program. I works on macOS and Windows as well as on Linux.
It is not necessary to install ApE to use it, you can use it from a folder on your desktop.
Go here to get ApE, click on the link for your OS as indicated below.
[ ape_download.png](https://jorgensen.biology.utah.edu/wayned/ape)
Introduction to ApE
[ ApE-20240924104257314.png](https://youtu.be/HTq91gJDSqk?si=5oT0bDhj6QIf4JwW)
[ ApE-20240924104312073.png](https://youtu.be/vDSiM2M_6JU?si=lpYg1SiVIt0a-mfq)
[ Pasted image 20240924104322.png](https://youtu.be/8n5yzd7hlXA?si=Aaoo4kIVvLdYnJX9)
How to linearize a circular sequence at a specific location.
- Locate the cursor at the desired location where you want to cut the vector. Make sure sequence is circular.
ApE_locate_linearization_site.png
- Select ApE Edit>"Linearize @ insert site" (see below).
ApE_linearize_insertion_site.png
The sequence is now linear beginning at the location of the cursor.