ApE - MetabolicEngineeringGroupCBMA/MetabolicEngineeringGroupCBMA.github.io GitHub Wiki

Downloading ApE

ape_download.png Use the download link for either Windows or MacOS as indicated in the image above. Get ApE here.

How to linearize a circular sequence at a specific location.

  1. Locate the cursor at the desired location where you want to cut the vector. Make sure sequence is circular.

ApE_locate_linearization_site.png

  1. Select ApE Edit>"Linearize @ insert site" (see below).

ApE_linearize_insertion_site.png

The sequence is now linear beginning at the location of the cursor.

ApE_linearized_sequence.png

https://youtu.be/zYqN9pF3ZVs