Protocol for making Synthetic Dextrose Minimal Medium (SD)

Also called Synthetic Minimal Glucose Medium or Synthetic Defined (SD). SD is a synthetic minimal medium containing salts, trace elements, vitamins, a nitrogen source (Bacto-yeast nitrogen base without amino acids), and glucose.

• 6.7 g/L Yeast Nitrogen Base (YNB) WITHOUT AMINOACIDS
• 20 g/L glucose
• 20 g/L Agar for solid medium

Mix the dry components in a bottle and add water. All components can be autoclaved together.

Alternatively, the medium can be made by combining stock solutions (1L):

• 100 mL 10x YNB
• 40 mL 50% Glucose
• Water and optionally agar to 1L

Ordinary tap water can be used for this since tap water was used to produce the yeast extract and the peptone in the first place. There is normally no need to set the pH.