Protocol for making Synthetic Dextrose Minimal Medium (SD)

Also called Synthetic Minimal Glucose Medium or Synthetic Defined (SD). SD is a synthetic minimal medium containing salts, trace elements, vitamins, a nitrogen source (Bacto-yeast nitrogen base without amino acids), and glucose.

• 6.7 g/L Yeast Nitrogen Base (YNB) WITHOUT AMINOACIDS
• 20 g/L glucose
• 20 g/L Agar for solid medium

Mix the dry components in a bottle and add water. All components can be autoclaved together.

Alternatively, the medium can be made by combining stock solutions (1L):

• 100 mL 10x YNB
• 40 mL 50% Glucose
• Water and optionally agar to 1L