This Spreadsheet has a detailed recipe.

Use:

• 2.028 g/L of the complete mixture
• 1.388 g/L of the Dropout - Histidin Uracile Leucine Trypthophan (-HULT)
• 1.308 g/L of the Dropout - Histidin Uracile Leucine Trypthophan Methionine (-HULTM)
• 0.640 g/L of the HULT (Histidin, Uracile, Leucine and Tryptophan only)

It can be practical to make stock solutions of aminoacids and nucleotides. Some aminoacids are not very soluble, using a slightly acid solution usually helps. The data below is from here. The data for uracile is from here.

AA	Solubility	final c in medium	times concentrated	Stock soln c	g/ 50 mL	µL/plate (20 mL)
-	g/L	mg/L	stock solution	g/L
HIS	41	80	100	8	0.4	200
URA	3.6	80	25	2	0.1	800
LEU	24	400	50	20	1.0	400
TRP	11	80	100	8	0.4	200
MET	33	80	100	8	0.4	200

It is practical to make 50 mL of solution for each component as this fit in our plastic syringes (for filtering).

1. Add about 20-30 mL of hot dH2O water in a 50 mL falcon tube
2. Add the amount specified in the second last column (g/ 50 mL)
3. Shake until dissolved.
4. Add water to 50 mL, the graduation on the Falcon tube has enough accuracy
5. Filter using syringe with 0.2 µM into a sterile Falcon tube
6. Store at room temperature, or the solutions may precipitate.