This Spreadsheet has a detailed recipe.

Use:

• 2.028 g/L of the complete mixture
• 1.388 g/L of the Dropout - Histidin Uracile Leucine Trypthophan (-HULT)
• 1.308 g/L of the Dropout - Histidin Uracile Leucine Trypthophan Methionine (-HULTM)
• 0.640 g/L of the HULT (Histidin, Uracile, Leucine and Tryptophan only)

It can be practical to make stock solutions of aminoacids and nucleotides. Some aminoacids are not very soluble, using a slightly acid solution usually helps. The data below is from here. The data for uracile is from here.

AA	Solubility	final c in medium	times concentrated	Stock soln c	g/ 50 mL	µL/plate (20 mL)
	g/L	mg/L	stock solution	g/L
HIS	41	80	100	8	0.4	200
URA	3.6	80	25	2	0.1	800
LEU	24	400	50	20	1.0	400
TRP	11	80	100	8	0.4	200
MET	33	80	100	8	0.4	200

It is practical to make 50 mL of solution for each component as this fit in our plastic syringes (for filtering).

1. Add about 20-30 mL of hot dH2O water in a 50 mL falcon tube
2. Add the amount specified in the secon last column
3. Shake until dissolved.
4. Add water to 50 mL, the graduation on the Falcon tube has enough accuracy
5. Filter using syringe with 0.2 µM into a sterile Falcon tube
6. Store at room temperature, or the solutions may precipitate.