Tween Myristic acid - MetabolicEngineeringGroupCBMA/MetabolicEngineeringGroupCBMA.github.io GitHub Wiki
Fatty acid requiring strains such as fas1- or fas2- needs myristic acid and tween in the medium.
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10 mL/L Tween 40 is Polyoxyethylene (20) sorbitan monopalmitate palmitate C16, providing palmitic acid (C16).
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0.460 g/L myristic acid (hmy), providing shorter C14 fatty acids.
Heat the bottle containing tween briefly in the microwave, 15 s. Pipette 2.5 mL (3 * 834µL) into 250 mL medium. Add 460/4 g myristic acid (powder) atutoclave