TBE - MetabolicEngineeringGroupCBMA/MetabolicEngineeringGroupCBMA.github.io GitHub Wiki

TBE

[protocol:] Revised 2012-02-01

Composition of 1x TBE buffer 89 mM Tris (pH 7.6) 89 mM boric acid 2 mM EDTA

1 liter of 25 x TBE: 270 g of Tris base (CAS# 37186) 121.14 g/mol 137.5 g of boric acid (CAS# 11280) 61.8 g/mol 100 ml of 0.5 M EDTA solution (pH 8.0) (CAS# 60004)

1 liter of 5 x TBE (GR): 54 g of Tris base (CAS# 37186) 27.5 g of boric acid (CAS# 11280) 20 ml of 0.5 M EDTA solution (pH 8.0) (CAS# 60004)

http://en.wikipedia.org/wiki/TBE_buffer

B6768-1KG 1000g 67 euros => (137.5/1000)*67 = 9 euro/L