Home - MetabolicEngineeringGroupCBMA/MetabolicEngineeringGroupCBMA.github.io GitHub Wiki
This wiki contains protocols and other information mostly of interest to the [mec]] group at CBMA and people working in the LGM lab. You can use the search box at the right to search for page titles. Search page content using this [method.
Basics
- Starting to work in the MEC group
- Maintaining lab notes
- How to mark your material
- Autoclaving
- Yeast and bacterial freezer stocks
- LGM
- Congresses
- Dept phone directory
- Teaching
Commonly used protocols
- Yeast transformation
- E. coli transformation
- PCR
- Microwave Yeast Colony PCR ◀️ This is the fastest protocol
- NaOH Yeast Colony PCR ◀️ This more work, but robust
- Yeast genomic DNA extraction with LiOAc SDS ◀️ This more work, but robust
- Agarose gel
- Plasmid miniprep with home made buffers
- Plasmid rescue from yeast to E. coli
- Making LB medium.
- Making YPD medium.
- Making defined SC or SD medium .
The Yeast Pathway Kit
- The Yeast Pathway Kit
- pYPKa cloning protocol
- Transcription Unit cloning protocol
- standard_primers
- primer design
- in silico assembly of pYPKa_A_ATF1
- in silico assembly of pTA1_TDH3_ATF1_PGI1
- Plasmids, Promoters & Terminators sequence files.