standard_primers - MetabolicEngineeringGroupCBMA/MetabolicEngineeringGroupCBMA.github.io GitHub Wiki
>577_crp585-557 (29-mer)
gttctgatcctcgagcatcttaagaattc
>578_crp42-70 (29-mer)
gttcttgtctcattgccacattcataagt
>468_pCAPs_release_fw (25-mer) 79.66 same as 560
gtcgaggaacgccaggttgcccact
>467_pCAPs_release_re (31-mer)
ATTTAAatcctgatgcgtttgtctgcacaga
>567_pCAPsAjiIF (23-mer)
GTCggctgcaggtcactagtgag
>568_pCAPsAjiIR (22-mer)
GTGCcatctgtgcagacaaacg
>1123_New775 (see primer 775 below)
gtgcaatgcggccgctGAC
>778_tp_Eco32I_rev (20-mer)
ggtaaatccggatTAATTAA
>494_tp_Eco32I_rev2 (29-mer)
ttcgccaattgattcaggtaaatccggat
>342_ORIR-1 (21-mer)
CCTTTTTACGGTTCCTGGCCT
>775_tp_ZraI_fwd (18-mer) we have found that primer 1123 works better (see above).
gcggccgctgacTTAAAT
>1715_pCAPs_release_fw shorter version of 468 (19-mer)
gtcgaggaacgccaggttg