2xPCR mastermix - MetabolicEngineeringGroupCBMA/MetabolicEngineeringGroupCBMA.github.io GitHub Wiki

Using a PCR master-mix saves time and improves PCR reproducibility, especially if many reactions are prepared. For a standard PCR reaction , half the volume should be 2xPCR mastermix. The standard recipe is as follows:

Component	µL	stock	2xPCR mastermix	final concentration in PCR reaction
Water	16	-	-	-
Buffer(xC)	5	10x	2x	1x
MgCl2(mM)	2	50 mM	4 mM	2 mM
dNTPs(mM)	1	10	0.4	0.2
Taq	1	100%	5.0%	2.5%
Total	25	-	-	-

See standard pcr protocol.

This google sheet contains the recipe above. Create a new tab named after the date of preparation in ISO 8601 format. Copy paste an old recipe and modify if necessary.