Lithium acetate-borate (LaBo) buffer is superior to both TAE and TBE for agarose gel electrophoresis of DNA. The conductivity is lower resulting in cooler runs at the same voltage or faster runs at similar temperature and higher voltage.

50x LaBo buffer (1L):

Compound	M(mol/L)	MW(g/mol)	m(g)	Compound	MW(g/mol)	m(g)
Lithium acetate (C2H3LiO2)	0.5	65.984	33	C2H3LiO2 x 2H2O	102.02	51.05
boric acid (H3BO3)	0.5	61.83	31
dH2O	---					to one L

Make sure the pH is between 6.5 – 7.0, if not add boric acid or lithium acetate.

Use the buffer at x1 concentration for gels and running buffer.

References:

• Faster, Even Cooler DNA Gels!
• https://openwetware.org/wiki/LAB_Media
• Improved DNA Electrophoresis in Conditions Favoring Polyborates and Lewis Acid Complexation