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Table 2 PCR strategies
Target product, template and primer numbers used for each element. Note that some targets are repeated.
| Table 2 | Product | Template | Primer1 | Primer2 |
|---|---|---|---|---|
| amp | pBR322 | 1113 | 987 | |
| amp | pUG35 | 1113 | 987 | |
| CEN_ARS | pSH65 | 1344 | 1343 | |
| HIS3 | p423GPD | 1352 | 1351 | |
| KanMX4 | pFA6a-GFPS65T-kanMX6 | 1350 | 1349 | |
| LEU2 | YIplac128 | 980 | 979 | |
| LEU2d | YIplac128 | 982 | 979 | |
| pBR | pBR322 | 1196 | 1195 | |
| TRP1 | YIplac204 | 1804 | 1347 | |
| TRP1 | YEplac112 | 1804 | 1347 | |
| URA3 | YEplac195 | 1346 | 1345 | |
| ΔCRP | pYPKpw | 978 | 977 | |
| ΔCRP | pTA1 | 978 | 977 | |
| 2µ | YEplac195 | 984 | 983 | |
| 2µ | YEplac181 | 984 | 983 |