TP26 Simply Cloning - MetabolicEngineeringGroupCBMA/MetabolicEngineeringGroupCBMA.github.io GitHub Wiki

A series of ten youtube videos by Andriy Nemirov describing a sub cloning experiment from planning to verification of the final construct. total run time is 65 minutes.

Simply Cloning - Chapter 1 - Planning 12:27

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Simply Cloning - Chapter 2 - PCR 06:39

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Simply Cloning - Chapter 3 - Vector Restriction Digest 02:52

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Simply Cloning - Chapter 4 - Gel Purification 11:47

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Simply Cloning - Chapter 5 - Insert Restriction Digest 02:55

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Simply Cloning - Chapter 6 - Ligation 06:10

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Simply Cloning - Chapter 7 - Transformation 08:02

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Simply Cloning - Chapter 8 - Plasmid Miniprep 06:05

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Simply Cloning - Chapter 9 - Clone Verification 05:20

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Simply Cloning - Chapter 10 - Clone Library 02:48

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Questions

Question 1: What is the cdseguid of the pSAT6-Bar vector?

Question 2: Why does he run the gel in Chapter 4?

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