I2PC INSTRUCT course: INTRODUCTION TO MODEL BUILDING 2022 - I2PC/scipion-courses GitHub Wiki
Model Building Course Material
-
Presentation
- Introduction to Model Building presentation
- Introduction to AlphaFold2
- Scipion model building tutorials:
- Specimens:
-
Practical working sessions:
Agenda
- June 22nd
- How to access to the remote desktop
- Importing data in Scipion
- Prediction of atomic structures with AlphaFold2 + Processing
- Map Preprocessing
- Model prediction by sequence homology
- Afternoon task proposal 1
- June 23rd
- Review (Kahoot)
- Rigid Fitting of Initial Models
- Flexible fitting
- Validation
- Afternoon task proposal 2
- June 24th
- Review
- Summary (Kahoot)
- Chain merge & symmetrization
- Analysis: contacts
- Comparison validation scores PDB structure
- Submission
- Summary
Data resources and IDs
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Human haemoglobin
- 3D map: emd_3488.map, available directory Data
- sequence:
- PDB reference: 1pbx (antartic fish: Pagothenia bernacchii)
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Human ion channel-like protein TACAN
- 3D map: emd-31482.map, available directory Data
- sequence:
- UniProt ID Q9BXJ8