Protocols library - glasgowlab/home GitHub Wiki
Wet lab protocols.
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Cloning
- PCR
- Site-directed mutagenesis
- PCR Mutagenesis (QuikChange® II)
- Gibson cloning
- Golden Gate Assembly
- Colony PCR
- Yeast colony PCR
- Competent cells preparation
- Transformations
- Yeast plasmid purification
- Zymoprep protocol
- Ethanol precipitation
Protein expression and purification
- Autoinduction protocol
- Common promoter induction levels
- Culturing mammalian cells
- Compiled yeast protein expression methods
- Generic LacI/GalR family protein purification protocol
- Benchtop nickel purification
- Ni regeneration protocol
- TEV protease cleavage protocol
- BirA biotinylation protocol
- Preparing FPLC
- FPLC fraction collector
- Cleaning the Sephacryl Column
Protein characterization
- SDS-PAGE protocol
- Coomassie staining protocol
- Western blotting protocol
- Run 1x Gel: Coomassie staining w/ Western blotting protocol
- Dot blot
- Pulldown protocol
- Circular dichroism
- Biolayer interferometry protocol
- Differential scanning fluorometry protocol
- Bradford assay
- ELISA
- Isothermal titration calorimetry
- Flow cytometry
HDX/MS
- LacI HDX: exchange protocol
- Packing a trap column
- Manual icebox LC/MS protocol for HDX samples
- HDX data analysis workflow
- Analyze intact mass on Compass
- Calibrate MS
- Detailed HDX/MS protocol with the LEAP system
- Quench buffers