Packing a trap column - glasgowlab/home GitHub Wiki

1. Open the column using wrenches, gently
2. Replace frits every ~5x or whenever the column runs at high pressure
3. Wash out with water, using a wash bottle
4. Reassemble half the column, first placing the metal ring on the plastic piece, and then the larger metal piece. It should be tightened, but not super tight
5. Screw on the white funnel on top and the syringe on the bottom.
6. The R2 resin* is in acetonitrile. Add it to the funnel and use the syringe to pull it through. When liquid enters the syringe, it’s probably packed.
7. Reassemble the column the rest of the way: metal ring, fret, large metal piece.

*R2 resin: Thermo Scientific POROS™ 20 R2 Reversed - Phase Resin