Ethanol precipitation - glasgowlab/home GitHub Wiki

DNA concentration with ethanol precipitation

  1. Make 3M sodium acetate buffer, pH 5.4.
  2. Add 1/10 volume NaAce to DNA.
  3. Add 3 volumes 96% ETOH (ice cold), vortex and put in -80 °C for 20-60 minutes.
  4. Centrifuge at 4 °C / 30 min / 3270 x g.
  5. Decant the supernatant.
  6. Add 300 µl 70% ETOH to pellet without resuspending the pellet, and spin for 5 more minutes.
  7. Decant supernatant and place tube at 37 °C, uncovered.
  8. Hopefully you can see the pellet. Remove the remaining solvent and dissolve the pellet in buffer.