Colony PCR - glasgowlab/home GitHub Wiki
- Place 50 ul of ddH2O in a 1.5 ml tube.
- Scrape a decent-sized colony from a plate and dissolve in the water.
- Heat at 95-98 °C for 5-10 min.
- Centrifuge at 12000 x g for 1 min.
- Use 10 ul of the supernatant as a template for the following PCR:
| Ingredient | Volume |
|---|---|
| template | 5 ul |
| oligo 1 | 1.25 ul |
| oligo 2 | 1.25 ul |
| Q5 2X MM | 12.5 ul |
| water | 5 ul |