Instruct virtual course on Single Particle Analysis by CryoEM 2020 - I2PC/scipion-courses GitHub Wiki
Image Processing for Single Particle Analysis
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Theory. Although not necessary for performing the practice, you may find useful the following theoretical explanations about the operations being performed: Slides
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Practice. For the practical work, we will use a few micrographs of apoferritin to reconstruct a 3A resolution structure of this macromolecule. If you have your own machine, you will need to install Scipion3 with the Xmipp, Cistem, Relion, Sphire, GCTF, and CryoSparc plugins (you will need to install the CryoSparc binaries before installing the plugin). You will find an explanation of the different parts of the workflow at:
Paper with an example workflow
You may download the solved project from here
Model Building Course Material
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Presentation
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Practical working sessions (General modeling workflow)
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Data resources and IDs
- BlastP (search for homologues)
- Human haemoglobin
- 3D map: emd_3488.map, available directory Data
- sequence:
- PDB reference: 1pbx (antartic fish: Pagothenia bernacchii)