Home - ryandkuster/EPP_575_RNA_25 GitHub Wiki

Welcome to the EPP_575_RNA wiki!

Course syllabus and content will be posted here as the course progresses. Be sure to check back soon!

Agenda

  • 💻 Day 1 - Wednesday, May 28
  • 💻 Day 2 - Thursday, May 29
  • 🌱 Day 3 - Friday, May 30
  • 🌱 Day 4 - Monday, June 2
  • 🌱 Day 5 - Tuesday, June 3

💻 - intro to command line
🌱 - intro to RNASeq

Before the workshop begins, please check out:

Getting started with UTIA Computing Resources

Installing relevant R, RStudio (posit), and R packages

The directory we are using on ISAAC-NG:

/lustre/isaac24/proj/UTK0386

Create a variable called `RNA' that points to your working directory for the labs:

echo 'export RNA=/lustre/isaac24/proj/UTK0386/analysis/${USER}' >> ~/.bashrc
source ~/.bashrc
mkdir -p $RNA
cd $RNA

The data used for the RNA-Seq analyses

CPK28-NLP7 module integrates cold-induced Ca2+ signal and transcriptional reprogramming in Arabidopsis

Raw data on NCBI

Raw data on ENA

TAIR10 Reference genome

Other useful links!

Link to Software Carpentry

Requesting resources with salloc

Most Commonly Used Commands

HPC Resources wiki