The aim of this practical session is to get your hands on real data (DNA/RNA) generated from two long-reads sequencing platforms:

• Oxford Nanopore (ONT) MinION
• Pacific Biosciences (PacBio) RSII

At the end of this practical, you should be able to:

• get an overview of the sequencing platforms, the sequencing method and their biological applications
• extract reads in standard fastq format from native HDF format specific to the technologies
• align the extracted reads to the reference genome/transcriptome
• use the extracted reads to assemble the genome and the transcriptome
• compare your assemblies to the reference for the analyzed species
• get an overview of downstream analysis you could apply on your assemblies
• understand the usage and theory behind the software you will be using here

Next

Learn about Pacific Biosciences: RSII .

Learn about Oxford Nanopore Technologies: MinION .

Find out what Biological material we will be working with.

Go to page with tips, how to do a proper File organization .

If you know everything above, jump to Checkpoint .

Go back to Table of content .