LAB7 - MetabolicEngineeringGroupCBMA/MetabolicEngineeringGroupCBMA.github.io GitHub Wiki

Summary:

• Run gel with colony PCR products from LAB6
• Inoculate 1 mL yeast cultures for plasmid rescue from liquid medium.
• Prepare solid LB Lennox medium for LAB8

1. Put a piece of gel in a square Petri dish
2. Load 8 µL of the the PCR product.
3. Run the gel for 15 - 20 min in TAE buffer.

While the gel is running, each group should prepare 250 mL solid LB Lennox medium in a 500 mL Schott bottle.

See if any of the previously prepared liquid YPD medium (LAB2) is still fresh? Inoculate 1 mL YPD medium in a 2 mL Eppendorf tube from the yeast plate using a sterile pipette tip.

This culture is for plasmid rescue next week. colored.png

ecoligfp.png