LAB5 - MetabolicEngineeringGroupCBMA/MetabolicEngineeringGroupCBMA.github.io GitHub Wiki

material#LAB5

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The objective of this task is to simulate the outcome of each of the five PCR product used to make the vector.

1. The primer numbers and template plasmids used can be found in Table#4 above.
2. The template sequences are available through links in Table 1.
3. The primer sequences for each primer number can be found in Table 3.
4. Simulate the PCR products using primer sequences and
5. Add the size and ldseguid checksum for each of the PCR products to the LAB5 tab in the course Google sheet.

1. Use the Assembly simulator (here) to join the five sequences.
2. Annotate your sequence with pLannotate
3. Add the size and cdseguid checksum for your plasmid as well as the sequence to the LAB5 tab in the Course Google Sheet.

Pick one isolated colony with a sterile pipette tip and inoculate a Petri dish with the the appropriate solid medium.

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