LAB1 - MetabolicEngineeringGroupCBMA/MetabolicEngineeringGroupCBMA.github.io GitHub Wiki
Summary:
- Plasmid miniprep using alkaline lysis
We will prepare four plasmids using alkaline lysis mini prep (see Table#3). These plasmids are the source for each of the five genetic elements. One plasmid is used as the source of two elements. The last column states why we need each particular plasmid.
| Table#3 | Group | Plasmid | Source for element |
|---|---|---|---|
| 1 | pBR322 | amp & pBR | |
| 2 | pYPKpw | Δcrp | |
| 3 | YIplac204 | TRP1 | |
| 4 | YEplac195 | 2µ |
We will use a homemade alkaline lysis plasmid miniprep protocol. Here is a short protocol for printing.
The teacher has prepared E. coli cultures in liquid or on solid medium beforehand.
Cultures with each plasmid were grown in or on LB with antibiotics for selection of the plasmids.
These videos show how to prepare plasmid DNA from E. coli using basically the same protocol.
