raw images - veeninglab/BactMAP GitHub Wiki

When importing a TIFF image using extr_OriginalStack(), the resulting dataset is a list of dataframes. Each dataframe in the list corresponds to one frame of your image stack and consists of three columns: x, y and values. Here, x and y stand for the x- and y-coordinate of a pixel of your image, while values is the greyscale value or intensity of this pixel.

You can use plotRaw() to view your image, or use any of the other functions using TIFFs to further process and visualize the intensity values.