Step 3: Create QIIME2 compatible metadata file - shenjean/diversity GitHub Wiki

QIIME2's metadata file is a tab-separated text file.

First row and first column must be sample-id. Subsequent columns is based on your metadata. Second row must start with #q2:types. Subsequent columns specify whether the corresponding column in the first row is categorical or numeric.

sample-id       gender  age           
#q2:types       categorical     numeric 
01bas   m       65     
01end   m       65      
01mid   m       65     
02bas   f       65      
02mid   f       65     
04end   f       58  
04mid   f       58      

Once you are happy with the file, save it as, for example, metadata.txt.

You can visualize your metadata file:

qiime metadata tabulate --m-input-file metadata.txt --o-visualization metadata.qzv