Muribaculum intestinale - mucosal-immunology-lab/bacterial-database GitHub Wiki
| Bacterial Information | Value |
|---|---|
| Taxonomy level | Species |
| NCBI Taxonomy ID | 1796646 |
| Phylum | Bacteroidetes |
| Family | Muribaculaceae |
| Genus | Muribaculum |
| Gram stain | Gram-negative |
| Oxygen requirements | Anaerobic |
| Spore-forming | No |
| Motile | No |
These bacteria are strictly anaerobic, and positive for α-galactosidase, β-galactosidase, N-acetyl-β-D-glucosamidase, alkaline phosphatase, leucyl glycine arylamidase and alanine arylamidase (Lagkouvardos 2016). Galactose can be degraded to form β-D-glucose-6-phosphate. Pectin can be depolymerised to D-galactopyranuronate. The main cellular fatty acids are anteiso-C15:0 (59.9%), C16:0 3-OH (8.3%), iso-C15:0 (5.6%), C14:0 (3.9%) and C15:0 3-OH (3.3%). Meso-diaminopimelic acid is absent.
After incubation on BHI haemin agar at 37°C for 3-4 days, cells were 1.9 – 2.6 μm in width and 3.0 – 3.4 μm in length (Park 2021). Colonies were circular, convex, entire, opaque, yellowish and 0.6 – 0.7 mm in diameter, with growth occurring at 28-41°C (optimum, 37°C), 0.5 – 1.0% NaCl (optimum, 0.5%), and pH 7.0 – 8.5 (optimum, pH 7.0 – 7.5). Positive reactions were detected for gelatine hydrolysis, mannose and raffinose fermentation, and negative reactions were detected for aesculin hydrolysis, indole production, reduction of nitrates, and urease activity (Park 2021).
Long term intake (12 weeks) of high sugar diet (65% carbohydrates – 85% glucose or fructose + 15% sucrose; 16.9% fat; 18.1% protein – 97.5% casein + 2.5% L-cysteine) significantly reduced Bacteroidetes, in particular M. intestinale, and increased Proteobacteria in the faeces of C57BL/6J mice (Do 2018).