Escherichia coli ATCC 8739 Adapts to the Presence of Sodium Chloride, Monosodium Glutamate, and Benzoic Acid after Extended Culture. - mauriceling/mauriceling.github.io GitHub Wiki

Citation: Lee, CH, Oon, JSH, Lee, KC, Lee, CH, Ling, MHT. 2012. Escherichia coli ATCC 8739 Adapts to the Presence of Sodium Chloride, Monosodium Glutamate, and Benzoic Acid after Extended Culture. ISRN Microbiology 2012, Article ID 965356.

Link to [Abstract], [Full Text] and [PDF].

Here is a permanent link to this [PDF] in my own archive.

We observed the adaptation of E. coli cultured in different concentration of food additives (sodium chloride, benzoic acid and monosodium glutamate), singly or in combination, over 70 passages. Adaptability over time was estimated by generation time and cell density at stationary phase. Polymerase Chain Reaction (PCR) / Restriction Fragments Length Polymorphism (RFLP) using 3 primers and restriction endonucleases each was used to characterize adaptation/evolution at genomic level and compared by Nei-Li Dissimilarity Index. Our results demonstrated that E. coli in every treatment had adapted over 465 generations. The types of stress were discovered to be different even though different concentrations of same additives were used. Genomic analysis by PCR/RFLP shows that the stress response in E. coli may be similar.