Characterization of Transcriptional Activities - mauriceling/mauriceling.github.io GitHub Wiki

Citation: Ling, MHT. 2025. Characterization of Transcriptional Activities. In Ranganathan, S., Cannataro, M., and Khan, A. M. (eds), Encyclopedia of Bioinformatics and Computational Biology (2nd Edition) Volume 5, Pages 408-422. Oxford: Elsevier. ISBN 978-0-323-95503-4.

Link to [Abstract]

Transcription is the first stage of gene expression, leading to the eventual determination of protein abundance and affecting metabolism. Hence, there is a need to measure and characterize transcriptional activities accurately. This article discusses the experimental techniques to characterize transcriptional activities from a single-gene approach (quantitative PCR) to high-throughput methods (microarray technology and next generation sequencing). Computational approaches to predict relative transcript abundance from sequence features and gene co-expression network will be described. As a proxy to protein / enzyme abundance, transcriptional activities are critical in developing simulatable biochemical models, which can then be used to test biological hypotheses prior to laboratory experimentation.