Ab Initio Whole Cell Kinetic Model of Thermus aquaticus Y51MC23 (taqTT26) - mauriceling/mauriceling.github.io GitHub Wiki

Citation: Tay, TZX, Dhinakaran, ML, Murugesu, S, Cinco, ANL, Srinithiksha, P, Ling, MHT. 2026. Ab Initio Whole Cell Kinetic Model of Thermus aquaticus Y51MC23 (taqTT26). Scholastic Medical Sciences 3(1): 01-04.

Link to [PDF].

Here is the permanent [PDF] and [dataset] links to my archive.

Thermus aquaticus is a thermophilic bacterium best known for its thermostable DNA polymerase used in polymerase chain reaction (PCR). This intrinsic thermostability of its proteins has also driven their use and consideration in other high-temperature engineering applications in molecular biotechnologies. Metabolic modelling using genome-scale or constraint-based models (GSMs) and kinetic models (KMs) can guide potential routes for investigations by providing predictive, in silico analyses. While GSMs are more often used, KMs enable a more comprehensive, dynamic visualization of the metabolic networks of the modelled organisms, and more naturally incorporate in silico gene modifications. Although a published GSM of T. thermophilus exists, there has been no GSMs or KMs of T. aquaticus to-date. Hence, a KM of T. aquaticus Y51MC23 was constructed in this study using ab initio approach by identifying enzymes from its published genome and identifying the corresponding reaction from KEGG. The resulting kinetic model, taqTT26; comprising of 1059 metabolites, 431 enzymes with corresponding transcriptions and translations, and 1522 enzymatic reactions; and can be a base template for future refinement.