Ab Initio Whole Cell Kinetic Model of Streptococcus thermophilus STH_CIRM_65 (stheVS26) - mauriceling/mauriceling.github.io GitHub Wiki

Citation: Verma, S, Nanthakumarvani, D, Perumal, LH, RajKumar, AA, Kang, CKN, Abul-Hasan, S, Ling, MHT. 2026. Ab Initio Whole Cell Kinetic Model of Streptococcus thermophilus STH_CIRM_65 (stheVS26). Acta Scientific Nutritional Health 10(2): 43-47.

Link to [PDF].

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Streptococcus thermophilus is a lactic acid bacterium, which is used in yogurt production, and known for its ability to produce folate, exopolysaccharides; thus, making it a highly valuable organism for food biotechnology and probiotic applications. Mathematical kinetic models, which extend beyond steady-state predictions in genome-scale models, are useful tools for directing metabolic engineering efforts. Although a genome-scale models of S. thermophilus is available, a comprehensive whole-cell kinetic model is lacking. In this study, we describe a simulatable kinetic model of S. thermophilus STH_CIRM_65, constructed in an ab initio fashion by locating enzymes from the genome sequence and mapping them to corresponding reactions in KEGG. The resulting model, stheVS26, encompasses 322 metabolites, 400 enzymes along with their transcription and translation processes, and 336 enzyme reactions. This model provides a foundational platform for the simulation and prediction of cellular behaviour, allowing for informed design decisions in metabolic engineering.