Ab Initio Whole Cell Kinetic Model of Streptococcus equi subsp. zooepidemicus SEZ13 (seqSA26) - mauriceling/mauriceling.github.io GitHub Wiki

Citation: Ali, S, Modankap, SH, Baylon, JLD, Dhingra, L, Mohamed Ali, N, Subramanian, P, Ling, MHT. 2026. Ab Initio Whole Cell Kinetic Model of Streptococcus equi subsp. zooepidemicus SEZ13 (seqSA26). Scholastic Medical Sciences 3(6): 02-04.

Link to [PDF].

Here is the permanent [PDF] and [dataset] links to my archive.

Streptococcus equi subsp. zooepidemicus (SEZ) is a Gram-positive opportunistic pathogen that is recognised for its natural capacity to produce hyaluronic acid through fermentation; hence, may be a candidate for metabolic engineering. Although both genome scale metabolic models (GSMs) and kinetic models (KMs) inform metabolic engineering strategies, KMs are more advantageous GSMs as it captures dynamic concentration changes. While a GSM of SEZ has been recently published, there is no KM of SEZ to-date. This study reports the ab initio construction of a whole-cell kinetic model for SEZ strain SEZ13 by identifying enzymatic genes from the annotated genome and mapping them into reactions in KEGG database. The resultant model, seqSA26, comprises 580 metabolites, 325 enzymes and 861 enzymatic reactions. Our results suggest that seqSA26 can be simulated and exhibit dynamic fluctuations of metabolite concentrations rather than flat lines, indicating a coherent network structure; therefore, establishing draft model for future refinements, including growth coupling and improved resource allocation simulations.