Ab Initio Whole Cell Kinetic Model of Lacticaseibacillus paracasei subspecies paracasei NTU 101 (lcaKKNC26) - mauriceling/mauriceling.github.io GitHub Wiki

Citation: Kang, CKN, Verma, S, Nanthakumarvani, D, Abul-Hasan, S, Perumal, LH, RajKumar, AA, Ling, MHT. 2026. Ab Initio Whole Cell Kinetic Model of Lacticaseibacillus paracasei subspecies paracasei NTU 101 (lcaKKNC26). Acta Scientific Microbiology 9(2): 03-08.

Link to [PDF].

Here is the permanent [PDF] and [dataset] links to my archive.

Lacticaseibacillus paracasei subspecies paracasei NTU 101 was isolated from the faecal matter of a healthy, breast-fed newborn infant in Taiwan. It has been shown to be potentially useful in gut health, and recognised by US FDA as “generally recognised as safe” leading to potential engineered probiotics applications. Mathematical kinetic models provide time-course profile of modelled metabolites, which can be used to guide metabolic engineering approaches. However, there is no kinetic model of L. paracasei to-date. In this study, we present a whole cell simulatable kinetic model of L. paracasei subspecies paracasei NTU 101, lcaKKNC26, constructed using ab initio approach by identifying enzymes from its published genome. The resulting model consists of 846 metabolites, 356 enzymes with corresponding transcriptions and translations, and 677 enzymatic reactions; which can be a baseline model for incorporating other cellular and growth processes, or as a system to examine cellular resource allocations necessary for engineering.