Generating Overviews - galizia-lab/pyview GitHub Wiki

Let's return to the VIEW tab, to create overviews (interactive analysis mode)

An overview is a false-color coded display of response patterns. How to calculate responses? That is given by the CTV-Method (in the blue box ). Method 22 calculates the difference between two time points: CTV_lastframe – CTV_firstframe (average of three frames each). Here, frame 20 is well before the stimulus, frame 30 is in the middle of the stimulus. Which measurement is used is selected in “Data Manager” (orange box ). Click on Generate(new) to get the false-color coded image – shown here to the right. Many settings allow for fine tuning (see green box , most in the section “output”). Play with the flags – for example, try the difference between frames 45 and 25. Most important: Signal_FilterSpaceFlag and Signal_FilterSpaceSize (for filtering); SO_individualScale (value 3 for min/max taken from image, value 0 for min/max taken from flags SO_MV_scalemax and SO_MV_scalemin). When you have created too many overviews and you are confused, close all windows (red box ). (Many flags have names with ‘SO_’ at the beginning, for ‘Show Overviews’. ‘LE_’ stands for ‘Load Experiment’ related flags. ‘CTV_’ stands for ‘Curve to Value’, since a time trace/curve is converted to a single value to show, for example, response strength). If you played around too much with flags, and want to go back to the initial flag settings, you can always reload the .yml file (arrow)

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