Components of ILTIS window - galizia-lab/pyview GitHub Wiki

The ILTIS panel allows to visualize single frames, time traces, and to select areas of interest. Chose the ROI type (arrow), and then click into the frame to select an area (circle in this case). Click multiple times to create more areas.

  • orange Image area for one selected frame (“current frame”), superimposing several measurements each with a different color if more than one measurement is selected.
  • yellow Area to adjust range for false-color code mapping, and to select the color table, for raw data display.
  • purple Area to adjust range for false-color code mapping, and to select the color table, for signals display (e.g. deltaF/F).
  • green List of measurements. The names are created from columns in the .list file, and can be adjusted (flag: LE_labelColumns).
  • orange Area to show time traces. Here, multiple measurements are chosen, and one ROI, showing the responses to different stimuli in one location.
  • purple Area to select ROIs. Change the name of the ROI here.
  • red (Arrow) Button (let’s call it “signal button”) to flip between Raw data and Signals (deltaF/F, or, in case of ratiometric data, the ratio (even if the image in the button still remains deltaF/F)

Now click on the signals button (arrow), and then select a few more ROIs. Then in the “select data” panel select a single measurement (for the tutorial, select measurement 7), and in the “ROI label” panel select all ROIs.

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Transfer Data to ILTIS Contents ILTIS: ROIs and traces