PRICE de novo assembly - ababaian/serratus GitHub Wiki

Here is how I ran price for Frank. I selected SAM records with CIGAR=151M and converted to fastq. The command-line options were suggested by the price author (Graham Ruby). This was my first quick-and-dirty attempt, no optimization attempted. Graham recommend read QC first using the method implemented in the price package but I didn't try that. The command line is messed up below, should have backslashes to continue the line but got lost in the wiki.

fq1=../fq/ERR2756788_1.fastq fq2=../fq/ERR2756788_2.fastq

virus=../fa/ERR2756788_151M.fa

mkdir -p ../price

/usr/bin/time -v -o price.time
$PriceTI -fpp $fq1 $fq2
600 90
-icf $virus
1 1 1
-nc 10
-dbmax 151
-mol 30
-tol 20
-mpi 80
-target 90 0
-trimB 0 1.5
-a 8
-o ../price/contigs.fa