Quantification of proteins localizations - UU-cellbiology/averageBasalBodies GitHub Wiki

This part quantifies protein distributions in the final averaged and registered stacks.

To generate intensity plot profiles along the center-to-periphery and longitudinal basal body axis
we used Macro-RadialPlotProfile_single.ijm. It uses XY coordinates obtained at the end of the last part.

In the batch mode, it can be run using Macro-RadialPlotProfile_batch.ijm
and it needs to be provided with the a4_averaged/rescaled_avrg/registered folder as an input.
(in the provided dataset reordered output is located in Figure6/Input folder)

You would need to install ImageJ's "Radial Profile Angle" plugin for this part,
available at the original site or in our backup.

The (radial-cylindrical) intensity profiles are stored as csv files
and later maxima positions and FWHM are automatically quantified using maxima_quantification.ijm macro.