(part 1) Step 5: average BB shape quantification - UU-cellbiology/averageBasalBodies GitHub Wiki

In the final step of this part, again, make sure that the Correlescence plugin version >= 0.0.7 is installed on your FIJI.

At this step quantification of the shape of the averaged BB (total protein stain channel) is performed.

Open FIJI and run s3c_estimate_diam.ijm macro.
The parameters are the same as in other macros.

Select a folder where the average volume (per cell) tif file is stored.

output

The macro creates a csv file with the same name as tif, containing
diameter and XY center coordinates of the BB (total protein channel).

what happens

Each slice of the final average image is fitted to the concentric circles template
in the same way as it is described in step 1.