[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 270286 sequences (20000131 bp)...
[M::process] read 270286 sequences (20000126 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 68492, 19, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (284, 847, 4476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12860)
[M::mem_pestat] mean and std.dev: (2003.65, 2461.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 17052)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (425, 438, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (375, 500)
[M::mem_pestat] mean and std.dev: (440.54, 16.00)
[M::mem_pestat] low and high boundaries for proper pairs: (350, 525)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (464, 2774, 4521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12635)
[M::mem_pestat] mean and std.dev: (3096.79, 2904.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 16692)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (575, 2592, 6716)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 18998)
[M::mem_pestat] mean and std.dev: (3254.77, 3031.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 25139)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 270286 reads in 89.108 CPU sec, 44.557 real sec
[M::process] read 270284 sequences (20000052 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 68444, 14, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (196, 562, 1283)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3457)
[M::mem_pestat] mean and std.dev: (660.15, 597.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4544)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (424, 438, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (372, 502)
[M::mem_pestat] mean and std.dev: (440.42, 16.46)
[M::mem_pestat] low and high boundaries for proper pairs: (346, 528)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2117, 4645, 7111)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17099)
[M::mem_pestat] mean and std.dev: (4539.00, 3153.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 22093)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (463, 1527, 3567)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9775)
[M::mem_pestat] mean and std.dev: (1919.00, 1861.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 12879)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 270286 reads in 89.273 CPU sec, 44.406 real sec
[M::process] read 270282 sequences (20000003 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 68822, 14, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (216, 352, 1019)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2625)
[M::mem_pestat] mean and std.dev: (646.44, 622.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3428)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (425, 438, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (375, 500)
[M::mem_pestat] mean and std.dev: (440.39, 16.10)
[M::mem_pestat] low and high boundaries for proper pairs: (350, 525)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (942, 2719, 7013)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 19155)
[M::mem_pestat] mean and std.dev: (3632.07, 2905.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 25226)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 270284 reads in 86.501 CPU sec, 43.013 real sec
[M::process] read 270286 sequences (20000125 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 68449, 20, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (310, 801, 1611)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4213)
[M::mem_pestat] mean and std.dev: (813.00, 628.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5514)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (425, 438, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (375, 500)
[M::mem_pestat] mean and std.dev: (440.38, 16.03)
[M::mem_pestat] low and high boundaries for proper pairs: (350, 525)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1440, 4319, 7098)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 18414)
[M::mem_pestat] mean and std.dev: (3865.40, 2885.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 24072)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 270282 reads in 85.831 CPU sec, 42.670 real sec
[M::process] read 270282 sequences (20000028 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 68354, 12, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (300, 357, 968)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2304)
[M::mem_pestat] mean and std.dev: (350.38, 205.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2972)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (425, 438, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (375, 500)
[M::mem_pestat] mean and std.dev: (440.54, 16.13)
[M::mem_pestat] low and high boundaries for proper pairs: (350, 525)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2311, 5445, 8096)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 19666)
[M::mem_pestat] mean and std.dev: (4932.50, 3106.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 25451)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (948, 1866, 2541)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5727)
[M::mem_pestat] mean and std.dev: (1686.91, 1035.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7320)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 270286 reads in 86.744 CPU sec, 43.085 real sec
[M::process] read 270284 sequences (20000142 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 68692, 21, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (404, 940, 6216)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17840)
[M::mem_pestat] mean and std.dev: (2780.18, 2936.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 23652)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (425, 438, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (375, 500)
[M::mem_pestat] mean and std.dev: (440.44, 16.17)
[M::mem_pestat] low and high boundaries for proper pairs: (350, 525)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1417, 3038, 6894)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17848)
[M::mem_pestat] mean and std.dev: (3874.29, 2902.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 23325)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 270282 reads in 85.959 CPU sec, 42.759 real sec
[M::process] read 270284 sequences (20000079 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 68787, 19, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (269, 656, 1670)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4472)
[M::mem_pestat] mean and std.dev: (807.27, 742.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5873)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (425, 438, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (375, 500)
[M::mem_pestat] mean and std.dev: (440.35, 16.02)
[M::mem_pestat] low and high boundaries for proper pairs: (350, 525)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (187, 3185, 7846)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 23164)
[M::mem_pestat] mean and std.dev: (3762.74, 3382.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 30823)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (464, 795, 4040)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11192)
[M::mem_pestat] mean and std.dev: (2313.91, 2405.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 14768)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 270284 reads in 85.361 CPU sec, 42.432 real sec
[M::process] read 270284 sequences (20000142 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 68830, 15, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (87, 344, 1673)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4845)
[M::mem_pestat] mean and std.dev: (896.38, 1132.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6431)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (424, 438, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (372, 502)
[M::mem_pestat] mean and std.dev: (440.14, 16.24)
[M::mem_pestat] low and high boundaries for proper pairs: (346, 528)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2391, 4220, 5905)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12933)
[M::mem_pestat] mean and std.dev: (4186.07, 2489.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 16447)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 270284 reads in 84.324 CPU sec, 41.957 real sec
[M::process] read 270286 sequences (20000146 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 68408, 17, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (425, 438, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (375, 500)
[M::mem_pestat] mean and std.dev: (440.52, 16.03)
[M::mem_pestat] low and high boundaries for proper pairs: (350, 525)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1403, 2630, 4619)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11051)
[M::mem_pestat] mean and std.dev: (3205.82, 2470.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 14267)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 270284 reads in 85.094 CPU sec, 42.321 real sec
[M::process] read 270284 sequences (20000097 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 68584, 25, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (314, 1043, 1597)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4163)
[M::mem_pestat] mean and std.dev: (705.86, 496.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5446)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (425, 438, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (375, 500)
[M::mem_pestat] mean and std.dev: (440.45, 16.10)
[M::mem_pestat] low and high boundaries for proper pairs: (350, 525)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2318, 4184, 8596)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 21152)
[M::mem_pestat] mean and std.dev: (4508.84, 3254.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 27430)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 270286 reads in 85.449 CPU sec, 42.524 real sec
[M::process] read 270284 sequences (20000003 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 68628, 21, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (560, 2268, 3571)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9593)
[M::mem_pestat] mean and std.dev: (2772.58, 2711.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 13619)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (425, 438, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (375, 500)
[M::mem_pestat] mean and std.dev: (440.48, 16.15)
[M::mem_pestat] low and high boundaries for proper pairs: (350, 525)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (409, 1708, 5211)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14815)
[M::mem_pestat] mean and std.dev: (2766.90, 2577.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 19617)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 270284 reads in 85.192 CPU sec, 42.341 real sec
[M::process] read 270286 sequences (20000081 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 69001, 13, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (359, 1374, 2995)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8267)
[M::mem_pestat] mean and std.dev: (1684.93, 1596.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10903)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (424, 438, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (372, 502)
[M::mem_pestat] mean and std.dev: (440.24, 16.26)
[M::mem_pestat] low and high boundaries for proper pairs: (346, 528)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1771, 4190, 6270)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15268)
[M::mem_pestat] mean and std.dev: (3509.00, 2129.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 19767)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 270284 reads in 86.123 CPU sec, 42.824 real sec
[M::process] read 270282 sequences (20000013 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 68744, 13, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (329, 513, 2042)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5468)
[M::mem_pestat] mean and std.dev: (898.67, 1218.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7181)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (426, 439, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (376, 501)
[M::mem_pestat] mean and std.dev: (440.58, 16.05)
[M::mem_pestat] low and high boundaries for proper pairs: (351, 526)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3726, 4810, 6812)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12984)
[M::mem_pestat] mean and std.dev: (4824.62, 2385.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 16070)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (412, 758, 2094)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5458)
[M::mem_pestat] mean and std.dev: (955.40, 862.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7140)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 270286 reads in 86.175 CPU sec, 42.845 real sec
[M::process] read 270284 sequences (20000099 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 68690, 14, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (322, 1088, 5865)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16951)
[M::mem_pestat] mean and std.dev: (2478.24, 2902.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 22494)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (425, 438, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (375, 500)
[M::mem_pestat] mean and std.dev: (440.42, 16.05)
[M::mem_pestat] low and high boundaries for proper pairs: (350, 525)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (47, 2722, 4810)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14336)
[M::mem_pestat] mean and std.dev: (2874.79, 3101.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 19099)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 270282 reads in 85.529 CPU sec, 42.571 real sec
[M::process] read 270284 sequences (20000037 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 68876, 14, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (425, 438, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (375, 500)
[M::mem_pestat] mean and std.dev: (440.30, 16.12)
[M::mem_pestat] low and high boundaries for proper pairs: (350, 525)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (199, 3114, 4389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12769)
[M::mem_pestat] mean and std.dev: (3162.43, 2638.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 16959)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 270284 reads in 84.685 CPU sec, 42.081 real sec
[M::process] read 270284 sequences (20000017 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 68746, 19, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (104, 507, 4517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13343)
[M::mem_pestat] mean and std.dev: (2371.38, 3000.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 17756)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (425, 438, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (375, 500)
[M::mem_pestat] mean and std.dev: (440.33, 16.09)
[M::mem_pestat] low and high boundaries for proper pairs: (350, 525)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1662, 4929, 7012)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17712)
[M::mem_pestat] mean and std.dev: (4614.32, 3064.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 23062)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (617, 1229, 3192)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8342)
[M::mem_pestat] mean and std.dev: (1623.60, 1515.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10917)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 270284 reads in 89.423 CPU sec, 44.449 real sec
[M::process] read 270284 sequences (20000110 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 69211, 25, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (425, 438, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (375, 500)
[M::mem_pestat] mean and std.dev: (440.22, 16.12)
[M::mem_pestat] low and high boundaries for proper pairs: (350, 525)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (153, 1650, 4598)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13488)
[M::mem_pestat] mean and std.dev: (2471.12, 2650.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 17933)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 270284 reads in 87.422 CPU sec, 43.494 real sec
[M::process] read 270284 sequences (20000054 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 68439, 16, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (301, 374, 669)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1405)
[M::mem_pestat] mean and std.dev: (340.73, 138.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1773)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (425, 438, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (375, 500)
[M::mem_pestat] mean and std.dev: (440.39, 16.06)
[M::mem_pestat] low and high boundaries for proper pairs: (350, 525)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2635, 4772, 6967)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15631)
[M::mem_pestat] mean and std.dev: (4220.25, 2822.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 19963)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (479, 1224, 2899)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7739)
[M::mem_pestat] mean and std.dev: (1144.88, 912.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10159)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 270284 reads in 87.470 CPU sec, 43.545 real sec
[M::process] read 270286 sequences (20000107 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 68626, 18, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (425, 438, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (375, 500)
[M::mem_pestat] mean and std.dev: (440.30, 16.10)
[M::mem_pestat] low and high boundaries for proper pairs: (350, 525)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (62, 2283, 5172)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15392)
[M::mem_pestat] mean and std.dev: (2936.56, 2942.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 20502)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (971, 1351, 3228)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7742)
[M::mem_pestat] mean and std.dev: (1940.73, 1656.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9999)
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 270284 reads in 84.472 CPU sec, 42.007 real sec
[M::process] read 270284 sequences (20000147 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 68824, 12, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (245, 349, 4380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12650)
[M::mem_pestat] mean and std.dev: (1658.60, 2340.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 16785)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (425, 438, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (375, 500)
[M::mem_pestat] mean and std.dev: (440.33, 16.12)
[M::mem_pestat] low and high boundaries for proper pairs: (350, 525)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (104, 585, 6645)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 19727)
[M::mem_pestat] mean and std.dev: (2527.17, 3081.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 26268)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 270286 reads in 81.112 CPU sec, 40.356 real sec
[M::process] read 270284 sequences (20000080 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 68622, 16, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (423, 574, 4272)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11970)
[M::mem_pestat] mean and std.dev: (2097.27, 2653.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 15819)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (424, 438, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (372, 502)
[M::mem_pestat] mean and std.dev: (440.04, 16.26)
[M::mem_pestat] low and high boundaries for proper pairs: (346, 528)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1838, 2787, 4810)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10754)
[M::mem_pestat] mean and std.dev: (3095.44, 2470.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 13726)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (460, 926, 2367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6181)
[M::mem_pestat] mean and std.dev: (1272.22, 1053.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8088)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 270284 reads in 81.245 CPU sec, 40.363 real sec
[M::process] read 270284 sequences (20000146 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 68841, 15, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (426, 439, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (378, 498)
[M::mem_pestat] mean and std.dev: (440.59, 15.79)
[M::mem_pestat] low and high boundaries for proper pairs: (354, 522)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (269, 697, 5115)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14807)
[M::mem_pestat] mean and std.dev: (2332.67, 2701.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 19653)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (426, 884, 4304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12060)
[M::mem_pestat] mean and std.dev: (1941.92, 2300.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 15938)
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 270284 reads in 83.597 CPU sec, 41.558 real sec
[M::process] read 270282 sequences (20000022 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 68747, 22, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (425, 438, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (375, 500)
[M::mem_pestat] mean and std.dev: (440.34, 16.07)
[M::mem_pestat] low and high boundaries for proper pairs: (350, 525)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2105, 3119, 4217)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8441)
[M::mem_pestat] mean and std.dev: (3056.24, 1806.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10553)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 270284 reads in 81.109 CPU sec, 40.339 real sec
[M::process] read 270284 sequences (20000072 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 68676, 10, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (370, 988, 3078)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8494)
[M::mem_pestat] mean and std.dev: (1696.89, 2293.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11202)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (425, 438, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (375, 500)
[M::mem_pestat] mean and std.dev: (440.25, 16.17)
[M::mem_pestat] low and high boundaries for proper pairs: (350, 525)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1803, 2306, 6457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15765)
[M::mem_pestat] mean and std.dev: (3415.60, 2950.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 20419)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 270282 reads in 81.636 CPU sec, 40.614 real sec
[M::process] read 53782 sequences (3979654 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 68543, 15, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (267, 995, 2568)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7170)
[M::mem_pestat] mean and std.dev: (1233.14, 1528.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9471)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (425, 438, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (375, 500)
[M::mem_pestat] mean and std.dev: (440.34, 16.04)
[M::mem_pestat] low and high boundaries for proper pairs: (350, 525)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (330, 3322, 4824)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13812)
[M::mem_pestat] mean and std.dev: (3280.73, 3108.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 18306)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 270284 reads in 82.576 CPU sec, 41.112 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 13775, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (424, 438, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (372, 502)
[M::mem_pestat] mean and std.dev: (440.38, 16.20)
[M::mem_pestat] low and high boundaries for proper pairs: (346, 528)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 53782 reads in 16.844 CPU sec, 8.241 real sec
[main] Version: 0.7.17-r1188
[main] CMD: bwa mem -t 2 -T 30 -M -R @RG\tID:AX227\tSM:AX227\tPL:Illumina ../test4_1_bwaindex/MSU7.fa ../test2_cutadapt/AX227_1.cleaned.fastq ../test2_cutadapt/AX227_2.cleaned.fastq
[main] Real time: 1072.196 sec; CPU: 2149.489 sec
[adiouf@node19 test4_2_bwamem]$